Review Article | Open Access

Volume 2024 |Article ID 0 | https://doi.org/10.34133/bdr.0047

Advances in Subcellular Accumulation Design for Recombinant Protein Production in Tobacco

Shi-Jian Song ,^1,2 Hai-Ping Diao,¹ Yong-Feng Guo,¹ Inhwan Hwang ^3,4

¹Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, China
²Beijing LifeScience Academy (BLSA), Beijing, China
³Department of Life Science, Pohang University of Science andTechnology, Pohang, Republic of Korea
⁴BioApplications Inc., Pohang, Republic of Korea

Abstract

Plants and their use as bioreactors for the generation of recombinant proteins have become one of the hottest topics in the field of Plant Biotechnology and Plant Synthetic Biology. Plant bioreactors offer superior engineering potential compared to other types, particularly in the realm of subcellular accumulation strategies for increasing production yield and quality. This review explores established and emerging strategies for subcellular accumulation of recombinant proteins in tobacco bioreactors, highlighting recent advancements in the field. Additionally, the review provides reference to the crucial initial step of selecting an optimal subcellular localization for the target protein, a design that substantially impacts production outcomes.