Research Article | Open Access

Volume 2024 |Article ID 0039 | https://doi.org/10.34133/bdr.0039

A Light-Driven In Vitro Enzymatic Biosystem for the Synthesis of α-Farnesene from Methanol

Xinyue Gui,^1,2,4 Fei Li,^2,4 Xinyu Cui,^2,3 Ranran Wu,² Dingyu Liu,² Chunling Ma,² Lijuan Ma,¹ Huifeng Jiang,^2,3 Chun You ,³ Zhiguang Zhu ^2,3

¹Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, The College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China
²Key Laboratory of Engineering Biology for Low-Carbon Manufacturing, TianjinInstitute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China
³University of Chinese Academy of Sciences, Beijing 100049, China
⁴These authors contributed equally to this work

Abstract

Terpenoids of substantial industrial interest are mainly obtained through direct extraction from plant sources. Recently, microbial cell factories or in vitro enzymatic biosystems have emerged as promising alternatives for terpenoid production. Here, we report a route for the synthesis of α-farnesene based on an in vitro enzyme cascade reaction using methanol as an inexpensive and renewable C1 substrate. Thirteen biocatalytic reactions divided into 2 modules were optimized and coupled to achieve methanol-to-α-farnesene conversion via integration with natural thylakoid membranes as a green energy engine. This in vitro enzymatic biosystem driven by light enabled the production of 1.43 and 2.40 mg liter−1 α-farnesene using methanol and the intermediate glycolaldehyde as substrates, respectively. This work could provide a promising strategy for developing light-powered in vitro biosynthetic platforms to produce more natural compounds synthesized from C1 substrates.